GlycatedAlbumin(Glycoalbumin) | |
| Manufacturer/Trade: | Exocell/Glycaben |
| CatalogueNumbers: | Glycaben:1003StripPlate |
| Methodology: | SandwichELISA |
| Summaryofprocedure: | Glycabenisanenzymelinkedimmunosorbentassayforthequantitativedeterminationofglycoalbumininhumanplasma.Itprovidesinformationconcerningglycemiccontrolanalogoustothatofferedbyglycohemoglobinmeasurements,exceptthattheproteinmeasuredisglycatedalbuminwhichhasashorterhalf-life. Theassaydependsuponamonoclonalantibody,A717,thatspecificallyrecognizestheglycatedmoietiesinglycatedalbumin.Inpractice,standardsorsamplesareaddedtoA717coatedplates(Glycaben).Theglycoalbuminintheseanalytesbindstooriscapturedbytheantibodyonthesolidphase,andunboundmaterialsarewashedaway.Asubsequentincubationwithanenzymeconjugatedantihumanalbuminantibodyleadstodetectionoftheboundmolecules.BoundconjugateisdeterminedcolorimetricallyafterreactionwithaTMBsubstratesolution. Byrelatingsampleresponsewiththatforthestandards,theconcentrationofglycoalbuminmaybedetermined.Aseparateanalysisoftotalalbuminallowstheresultstobeconvertedtopercentoftotalalbuminthatisglycated. |
| SpecimenRequired: | 50ulcitrateorEDTAtreatedplasma |
| AssayRange: | 0.1-3mg/mlglycatedalbumin |
| Sensitivity: | 0.1mg/ml |
| Precision: | Intra-andinterassayprecisionofsampleswithintheassayrangehaveaC.V.<10%. |
| Specificity: | ThemonoclonalantibodyA717isspecificforglycatedalbumin,anddoesnotcross-reactwithotherproteinsthatoccurinhumanplasmaincludingnon-glycatedalbumin.Theenzymeconjugatedantibodypreparationisspecificforhumanalbumin. |
| ExpectedValues: | Plasmafromnormalnon-diabeticindividualhave0.4-2.4%glycatedalbuminwhereasthatfromuncontrolleddiabeticswillbe>2.5%. |
 ©2012ExocellInc. | |
