TheCreatinineCompanion | |
| Manufacturer/Trade: | Exocell/TheCreatinineCompanion |
| CatalogueNumbers: | 1012StripPlate |
| Methodology: | ChemicalAnalysis |
| Summaryofprocedure: | TheCreatinineCompanionisachemicalassaydesignedtomonitorurinarycreatinineinaconvenientmicroplateformat.Itisintendedasa"companion"forExocellurinaryalbuminassays. TheassayisbasedupontheJaffe"reactionofalkalinepicratewithcreatinine.Standardsorsamplesareaddedtoamicroplate,andalkalinepicratereagentisadded.Absorbanceat500nmisdeterminedaftera10minuteincubationonthebenchtop.Subsequently,anacidsolutionisadded,andabsorbanceisagaindeterminedaftera5minuteincubation.Thedifferencebetweentheseabsorbancevaluesisdirectlyproportionaltothethecreatinineconcentration,andastandardcurveisgeneratedfromtheresponsetothestandards.Unknownsamplesareevaluatedbycomparingresponsetothestandardcurve.Theassaymaybecompletedinlessthan30minutes. Theassayservesasanormalizationprocedureforotheranalytesinurinesamples,particularlyforurinaryalbumin(1,2).Itallowsforameasureofkidneyfunctionbasedonaspoturinesampleratherthantheusual24hourpool. |
| SpecimenRequired: | Urine,20ul |
| AssayRange: | 10-100ug/mlor1-10mg/dL |
| Precision: | Intra-andinterassayprecisionofsampleswithintheusefulrangehaveaC.V.<10%ofthemean. |
| References: | 1.Bennet,P.H.et.al.Screeningandmanagementofmicroalbuminuriainpatientswithdiabetismellitus.Am.J.KidneyDis.25:107,1995. 2.Warram,J.H.et.al.Effectofdurationoftype1diabetiesontheprevalenceofstagesofdiabeticnephropathydefinedbyurinaryalbumin/creatinineratio.J.Am.Soc.Nephrol.7:930,1996. |
 ©2012ExocellInc. | |
