DTNB-Thiols | |
| Manufacturer/Trade: | Exocell/DTNB-Thiols |
| CatalogueNumbers: | 1021 |
| Methodology: | ChemicalAssay |
| Summaryofprocedure: | Thethiolsassaykitmeasuressulfhydrylgroupswiththethiolreagent5-5"-dithiobis[2-nitrobenzoicacid](DTNB),whichformsthe5-thionitrobenzoicacidandamixeddisulfide.Underconditionsofoxidativestress,freesulfhydryldecreasesanddisulfidesincrease.DeterminationofthefreethiolconcentrationinBIOLOGicalsamplesreflectstheABIlitytodetoxifylipidandotherperoxides.Exocell"sDTNB-Thiolsassaycanbeusedwithaspectrumofbiologicalsamplesincludingbodyfluids,tissueandcellspecimens. Tocompletetheassay,aliquotsofstandardorsamplearereactedwithrehydratedDTNBreagentinmicrotiterwells.Absorbanceisreadat405nmusingareferencefilterof~630nm.Concentrationinsamplesisdeterminedfromthestandardcurve. |
| SpecimenRequired: | 50uL |
| AssayRange: | 0-56.9nmole/well |
| Precision: | Intra-andinter-assayprecision<5% |
 ©2012ExocellInc. | |
