rGlycatedAlbumin | |
Manufacturer/Trade: | Exocell/Glyco-AlbuminR |
CatalogueNumbers: | 1017StripPlate |
Methodology: | SandwichELISA |
Summaryofprocedure: | TheGlyco-AlbuminRassayisanELISAdesignedspecificallyforusewithratserumsamples.Inpractice,seraarediluted1:11tofallwithintheusefulrangeoftheassay. Theassaydependsuponthebindingofratglycatedalbumininstandardorsampletospeciallytreatedmicrotiterwells.Boundanalyteisdetectedwithananti-ratalbuminantibody-HRPconjugate.Afterafinalwash,boundconjugateisdetectedusingachromogenicsubstrate(TMB)forperoxidase,andcolorintensityisdirectlyporportionaltoglycatedalbuminconcentration.Typicalglycatedalbuminvaluesliebetween0.1and0.6mg/dL,abutnormalvaluesmayvarywithdifferentstranisofrats. |
SpecimenRequired: | Serum:20uL |
AssayRange: | 0.1-0.6mg/mL |
Precision: | IntraassayandinterassayprecisionforsampleswithintheusefulrangeoftheassayhaveaC.V.<10%ofthemean. |
©2012ExocellInc. |