请使用支持JavaScript的浏览器!
主营:免疫测定试剂盒
℡ 4000-520-616
℡ 4000-520-616
Exocell/mCollagen IV/1014 Strip Plate
产品编号:1014StripPlate
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Exocell
公      司:Exocell Inc.
公司分类:
Exocell/mCollagen IV/1014 Strip Plate
商品介绍
品牌介绍

Ethos是设计,开发和制造高品质,易于使用的肾脏疾病诊断试剂盒和试剂的全球领导者我们的产品可帮助对全球医疗保健产生影响的科学,医学和实验性肾脏病研究人员。


目的 –我们存在通过为科学家和从业者提供诊断工具来推动肾脏病医疗创新

愿景 -我们将成为肾脏疾病研究和临床分析的领先提供商

任务 –我们设计,开发和制造准确,可重复且易于使用的化验。


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔

mCollagenIV


Manufacturer/Trade:Exocell/CollagenIVM
CatalogueNumbers:1014StripPlate
Methodology:CompetitiveELISA
Summaryofprocedure:

CollagenIVMisanindirectcompetitiveELISAdesignedtomeasuretypeIVcollageninBIOLOGicalandtissueculturespecimensofmurineorigin.Thekitalsoworkswithratspecimens.Theassaymaybecompletedina"normalsensitivity"mode,withausefulassayrangeof0.156-10ugcollagenIV/mL.Aslightlydifferentprocedure,the"enhancedsensitivity"modetypicallyyieldsausefulassayrangeof0.02-2.5ug/mL,andissensitiveenoughtodetectcollagenIVinurinespecimens.

Tocompletetheassay,sampleandrabbitanti-collagenIVantibodyareaddedtomurinecollagenIVcoatedwells.TheantibodyinteractsandbindswiththecollagenIVimmobilizedtothestationaryphaseorwiththeantigeninthefluidphase,hencethenotionofcompetitivebinding.Afterasuitableincubationperiod,theplatesarewashed,andananti-rabbit-HRPconjugateisusedtodetectboundrabbitantibody.Afterwashing,onlytheantibody-conjugateboundtothestationaryphaseremainsinthewell,andthisisdetectedusingachromogenicreaction.ColorintensityisinverselyproportionaltothelogarithmofcollagenIVinthefluidphase.Theassaymaybecompletedinlessthan3hours.

The"enhancedsensitivity"modeiscompletedbyplacingthesamplesandanti-collagenIVantibodyintotubesandincubating2-24hours.Here,theantibodycanonlyreactwiththecollagenIVofthefluidphase.Subsequently,aliquotsofthemixtureareaddedtotheplatewhereremainingfreeantibodycanreactwiththeantigenofthestationaryphase.Theboundantibodyisdetectedasdescribedabove.

SpecimenRequired:50-100uLCellCultureExtract,Urine
AssayRange:

NormalSensitivity:0.156-10ug/mL

EnhancedSensitivity:0.02-2.5ug/mL

Precision:IntraassayandinterassayprecisionforsampleswithintheusefulrangeoftheassayhaveaC.V.<10%ofthemean.



©2012ExocellInc.