UniversalTBARs | |
| Manufacturer/Trade: | Exocell/UniversalTBARS |
| CatalogueNumbers: | 1020 |
| Methodology: | ChemicalAssay |
| Summaryofprocedure: | TheTBARsassaykitmeasuresmalondialdehyde(MDA),areactivecompoundformedfromlipidperoxidesthataregeneratedunderconditionsofoxidativestress.Oxidativemodificationoflipidsoccurswithagingandvariousdiseases,andincreasedoxidativestressisassociatedwithdiabetesanditscomplications.Cellularexposuretooxidativestressgivesrisetohighlyreactiveandunstablelipidhydroperoxides.DecompositionofunstableperoxidesderivedfrompolyunsaturatedfattyacidsleadstotheMDAformation.MDAreactswiththiobarbituricacid(TBA)toformanadductthatcanbemeasuredspectrophotometricallyorfluorometrically,thelatterprovidinggreatersensitivity.DeterminationofTBARsinBIOLOGicalsampleshasbecomethepreferredmethodforassessinglipidperoxidationandoxidativestress.Exocell"sTBARsassaycanbeusedwithaspectrumofbiologicalsamplesincludingbodyfluids,tissueandcellspecimens. Tocompletetheassay,standardorsampleisreactedwiththiobarbituricacidandheatedinthepresenceofaceticacid.Aftercoolingandclarificationbycentrifugation,aliquotsareplacedintomicrotiterwellsofablackplateandreadinafluorometeratexcitation484nm,emission530nm,orintoaplasticplateforreADIngat530-540nminaspectrophotometer.MDAconcentrationinsamplesisdeterminedfromthestandardcurve. |
| SpecimenRequired: | 100uL |
| AssayRange: | 0-30nmolefluorometrically 0-300nmolespectrophotometrically |
| Precision: | Intra-andinter-assayprecision<8% |
 ©2012ExocellInc. | |
